BIOCHEMICAL STUDIES OF PHYLOGENY 127 



Species Creatine Arginine 



Arenicola marina 

 Audouinia tentacidata 

 Clymene himbricoides 

 Dasybranchus caducus 

 Glycera convoluta 

 Lineus marinus 

 Lnmbriconereis 

 Marphysa sanguinea 

 Nephthys hombergi 

 Nereis diversicolor 

 Sabella pavonina 

 Scolophus armiger 

 Lumbricas terrestris 

 Hirudo medicinalis 

 Phascolosoma elongatum 

 Sipuncidus nudus 

 Ascaris himbricoides 

 Actinia equina 

 Anemonia sulcata 

 Calliactis parasitica 

 Halicho?idria panicea 

 Hymeniacidon caruncula 

 Thetia lyncurium 

 Tetrahymena geleii 



The authors conclude that the very wide distribution of 

 creatine does not allow one to come to any conclusion concerning 

 its phylogenetic importance and in particular the presence of 

 creatine in the echinoderms in no way indicates an affinity or 

 relationship with the vertebrates. This view is supported by 

 Ennor and Morrison (1958) in their review of the biochemistry of 

 phosphagens and related guanidines. 



What conclusion can be drawn with regard to the distribution 

 of phosphagens in the animal kingdom? The first conclusion is 

 that there is certainly no simple cleavage of the animal kingdom 

 into vertebrates with CP and invertebrates with AP. Instead it is 

 clear that both CP and AP are found throughout the invertebrates. 

 The second conclusion is that one cannot base any phylogenetic 



