THE BACTERIAL ENDOSPORE 



45 



8 



PH 



8 12 



Time in hours 



Fig. 7. Reversal by succinate of the inhibition of sporulation of B. cereiis T. 



by a-picoHnic acid. 



In Fig. 7 we show the effect of succinic acid on the inhibition 

 of sporulation by a-picoHnic acid. This shows that succinic 

 acid reverses the inhibition, even when it is added as late as five 

 hours after growth starts. In view of this, we have tested a large 

 number of other acids, including some amino acids, for their 

 ability to reverse this inhibition. The results are shown in 

 Table II and Table III. It is to be noted that all of the inter- 



TABLE II 



EFFECT OF AMINO ACIDS ON THE INHIBITION OF SPORULATION BY 



a-PICOLINIC ACID 



Addition of a-Picolinic acid (1.2 X 10~^ M) 1 mg/ml 

 to 1 mgjml of 



Aspartic acid 



Asparagine 



Alanine 



Arginine 



CitruUine 



Cysteine 



Cystine 



Diaminopimelic acid 



Glutamic acid 



Glutamine 



Glycine 



Histidine 



/S-Hydroxyglutamic 



References p. 59 



Sporulation 



+ 

 + 



