THE BACTERIAL ENDOSPORE 



49 



As other possible inhibitors of sporulation, we have tried the 

 esters of acids in the tricarboxylic acid cycle. The results are in- 

 dicated in Table VL In this table, failure to get spores shows that 



TABLE VI 



EFFECT OF SOME ESTERS ON SPORULATION OF B. CereUS T 



the ester is serving as an inhibitor whereas a normal spore crop 

 shows that no such inhibition takes place. Ethyl pyruvate, 

 diethyl succinate, ethyl malonate, and diethyl oxalacetate in- 

 hibited sporulation, but ethyl acetate, triethyl citrate, ethyl 



8 



pH 



8 12 



Time in hours 



Fig, 8. The effect of diethyl malonate (1.3 x IO-2 M) on the pH and 

 sporulation of a culture of B. cereus T. 



References p. 59 



