92 



H. HALVORSON et al. 



L-ALANINE 



GERMINATING AGENTS 



Fig. 8. Route of L-alanine for germination. 











Controls (i DPA) 



30 



60 



90 120 



Minutes 



Fig. 9. Stimulatory effect of dipicolinic acid on alanine deamination by 

 crude spore extracts. Dialyzed crude extract (2.1 mg protein) of heat-shocked 

 spores incubated at 30 in Conway diffusion units containing 0.5 /imole 

 of DPN, and, where indicated, 50 //moles of L-alanine and/or 40 /tmoles 

 of DPA in 0.067 M phosphate buffer, pH 7.5. 



