IN VIVO ia'N(n"I()N Ol- sriiCULLl'LAR ['ARTICLES 



I I 



ATP-ase 

 A5'P-ase 

 Alk. P-ase 



Glucose-6-P-asc, 

 Esterase 



<4_: Oxidative 

 f~^ enzymes, 



ATP-ase 



Golgi 



Lipide 



Acid P-ase 

 DPNH-diaph. 



Fig. 9. Schematic representation of a parenchymatous cell of rat liver, with adjacent Kupffer 

 cell (stellate shape). Note orientation of mitochondria (long axes oriented in sinusoid- 

 canaliculi directions). The pericanalicular arrangement of Golgi apparatus, dense bodies, acid 

 phosphatase bodies and esterase bodies is shown; the uncertainty concerning their inter- 

 relations is indicated by question marks. Abbreviations: Acid P-ase, acid phosphatase; A5 P-ase, 

 5'-nucleotidase; ATP-ase, adenosine triphosphatase; Alk. P-ase, alkaline phosphatase; Glucose- 

 6-P-ase, glucose-6-phosphatase; DPNH-diaph, DPNH-tetrazolium reductase; DNA, deoxy- 

 ribonucleic acid; RNA, ribonucleic acid; Arg. fibers, argyrophylic fibers. 



biochemical assay of isolated fractions and electron microscopy, but also on the 

 results of specific staining methods. 



SPECIFIC CYTOCHEMICAL STAINING METHODS 



We will refer to only one cytochemical staining method tor nonenzymatic 

 constituents, the Feulgen method for DNA (for a fuller discussion of this and 

 other methods see refs. 26, 30). It has recently been applied to a problem which 

 may be of considerable interest to in vivo function. Even it it pro\c of less 

 physiological interest than now appears, the work illustrates the possibilities ol 

 quantitation with some of these staining methods. It is agreed by most that, with 

 but few exceptions, the DNA content of cells is generally constant. Yet, at least 



