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Fig. in. ATPase activitx' in frozen section of rat liver fixed in cold formol-calciuiii. [From 

 (33). I Incubated for 15 min. at 37°C in incubation medium of Wachstein anil Meiscl (57), 

 with the addition of 2.5 X i« '* m cysteine hytlrochloride. Portal triad in upper right. Bile 

 canaliculi of peripheral cells are wider and show more intense ATPase activity than centro- 

 lobular cells (lower left); cf. fig. 20. X 420. 



Fig. II. Bdectron micrograph of section of rat liver. (From (9).] Tissue hxed in cold buf- 

 fered osmium tetroxide for 7 min., then incubated in ATPase medium for 30 min. at 37°C. 

 Note nuclei, mitochondria, and vacuolated cytoplasm. The reaction ])roduct is all i)resent inside 

 the bile canaliculus. X 375"- 



Fig. t2. Klectron micrograph of Iner treated as in lig. 11. | I'lom (q).| Note pricijiitate in 

 the plasma membrane surrountling bile canaliculus ;uul at tips of some microvilli. There is no 

 precipitate in the adjoining regions of the plasm. 1 meinbr.me. X I9>-""- 



Fig. 13. ATPase activity in kidnex of a rat niadi' nephrotic by injection of aminoiuicleoside. 

 [From (51).] In the proxim.il tubule, cut longitudiualK', the indented cell membranes show 

 marked ATPase activity. Activity is also high in the membranes of the capillary endothelium 

 (on either side of the proximal tubule). X 7'^"- 



13 



