IN VIVO ll'NClION OF SUBCELLULAR PARTlCLliS 



19 



^%^'^ 4< > w^ '->*-^^ .r/: 





V--,- 



DPNH LDH /3HB 



a^^ 



LIPASE- 

 IGM£NT--N.SXhE7=- 



GLYCOGEN 



9 



SDH ATP-Qse 



(+) 



uni 



form 



TPNH(prel.) 

 IDH (prel.) 

 MAO (?) 



++++ ++(+) + + (+) 



+ + 



+ +++ 



Fig. 20. Diagrammatic representation of a hepatic lobule of the rat. 



Upper left quadrant, a photograph shows a portion of the hepatic lobule stained to demon- 

 strate ATPase activity in the bile canaliculi. [From (33).] The quantitative dififerences between 

 centrolobular cells and perii)heral cells are indicated by plus marks ( + ) and by schematic 

 representation of the cytologic structures. Abbreviations: ATP-ase, adenosinetriphosphatase; 

 A5'P-ase, 5'-nucleotidase; Alk. P-ase, alkaline phosphatase; Acid P-ase, acid phosphatase; G-6- 

 P-ase, glucose-6-phosphatase; DPNH, DPNH-tctrazolium reductase; LDH, lactic dehydrogenase- 

 DPNH-tetrazolium reductase; BHB, beta-hydroxybutyric dehydrogcnase-DPNH-tetrazolium 

 reductase; SDH, succinic dehydrogenase; TPNH, TPNH-cytochromc c reductase; IDH, isocitric 

 dehydrogenase-TPNH tetrazolium reductase; MAO, monamine oxidase; N.S.ChE., non-specific 

 cholinesterase; prcl., preliminary observations. KupfTer cells are indicated where they contain 

 high levels of the enzyme activities or large numbers of the cytological structures drawn. 

 (Evidence obtained in our laboratory since this diagram was drawn shows that TPNH-tetra- 

 zolium reductase activity is also higher in the centrolobular than in the peripheral cells.) 



