STKUCTIKAL lACTOKS IN POLVM kKIZATION 



53 



pciul u[K)n the geometry and 

 pattern of the charged surface. 

 Again an avenue for coinhined 

 theoretical and experimental at- 

 tack, upon the mechanism ot 

 matrix action is evident, al- 

 thouirh the hhrin surface is un- 

 douhtedly too complex to 

 qualify as a subject for detailed 

 investigation. 



In addition to the matrix ef- 

 fect as seen in the supernatant 

 phase, quinone derivatives were 

 deposited in the protein itself. 

 Fractionation of these products 

 permitted recognition of at least 

 two modes of association be- 

 tween polymers and matrix; 

 first, those products bound by 

 weak forces (presumably H- 

 bonds in most cases) which 

 were extracted readily by solu- 

 tions of urea or LiCl; and sec- 

 ond, those products held by 

 covalent bonds, (quinone tan- 

 ning) thus solubilized only by 

 proteolysis (with trypsin). The 

 latter were isolated as addition 

 compounds with water-soluble 

 peptides. Water, urea and LiCl- 

 insoluble products soluble in 

 KOH, but not KHCO3, pre- 

 sumably contained weakly acidic 

 phenolic hydroxyl groups which 

 would contribute to the total 

 strength of hydrogen bonds. 



The spectral characteristics of 

 polycjuinones formed in various 

 media, without matrix, with 

 fibrin, or with collagen have 

 been summarized in table 6, 

 where it may be seen that 



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