ii6 



SUBCELLULAR PARTICLES 



Fig. 2. Electron micrograph showing an array of Golgi membranes at higher magnification. 

 Pairs of membranes are joined at the ends (arrow) indicating that the membranes bound 

 flattened sacs of considerable extent. Expansion of an intramcmbranous space forms a large 

 vacuole at V. Smaller vesicles are also present (v). The bar represents 0.5 micron. X 55, 000. 



region of the epididymal cells in the unfixed state (4). Upon disruption of the 

 cells in salt-containing media, structures were released which retained for several 

 hours the configuration and optical properties peculiar to the in situ formations 

 (4). In collaboration with Dr. Walter C. Schneider, it was found that this par- 

 ticulate material, which we referred to as Golgi material or Golgi substance, 

 could be isolated by centrifugation in a sucrose density gradient (22, 23). 



