122 



SUBCELLULAR PARTICLES 



* 4^ * 



Fig. 7. Electron micrograph of isolated Golgi material. The G fraction, obtained as indicated 

 in the text, was fixed in cold i per cent osmium tetroxide containing appropriate concentrations 

 of sucrose and sodium chloride, and centrifuged at high speed. The resultant pellet was washed, 

 dehydrated, embedded in plastic, and sectioned (13). Characteristic arrays of Golgi membranes 

 are seen (GM). Expansion of the intramembranous spaces to form large vacuoles (V) is a 

 frequent occurrence, as is fragmentation of the membranes to form small granules (g) reminis- 

 cent of the vesicles (v) seen in the intact cell (figs, i and 2). To a large extent, the impression 

 of heterogeneity results from variations in the plane at which the Golgi material was sectioned. 

 The bar rejiresents 0.5 micron. X 33, 000. 



