124 



SUBCELLULAR PARTICLES 



% Ji 



^ 



'/ -^'s 





* < ^ 'I X 



Fig. 9. Electron micri)graph of the UG fraction obtained as iniiicatcxi in the text and treated 

 as described in the legend for figure 7. Organized arrays of membranes do not appear in this 

 fraction. The profiles of many thin-walled vesicular structures, usually cut somewhat tangen- 

 tially, are seen (VS). The intracellular sites of origin of the material in this fraction cannot be 

 determined with certainty (see text). The bar represents 0.5 micron. X 33,000. 



chloride (Golgi and Under-Golgi^ ) or in 0.88 m sucrose alone (mitochondria 

 and microsomes). All other values refer to fractions exposed to 0.34 m salt. 



1 Golgi material isolated at this salt concentration showed remarkably good jireservation of 

 structural details. However, the tractions are contaminated with mitochondria and with rough- 

 surfaced membranes derived from the ergastoplasmic elements of the intact cell (fig. i). 



