136 



SUBCELLULAR PARTICLES 



1.23 



1.24 



1.25 



Fig. 5. Density-equilibration in sucrose-D^O gr.ulicnt. Washed total mitochnndrial fraction 

 evenly distributetl in a gradient of sucrose in D^O and centrifuged for 25 hr. at 39,000 rpm 

 in SW-39 rotor of Spinco Model L centrifuge. For technical details, see de Duve et al. (25). 

 It should be noted that a negligible separation between mitochondria, lysosoines and uricase 

 particles obtains in similar experiments performed in H^O-sucrose solutions. 



