BIOCHEMICAL PROPKRTIES OF THE ISOLATED NUCLEUS 



193 



Table 2. Effect of supplementary dna's in restoring c -alanine uptake in 



DNAase-TREATED NUCLEI 



the gift of these dinucleotides.) Among the dinucleotides tested was adenyUc- 

 adenylic dinucleotide, chromatographically purified after rapid acid hydrolysis of 

 yeast RNA; this compound failed to promote alanine uptake in DNAase-treated 

 nuclei. This observation takes on added interest when it is compared with the 

 previous finding that polyadenylic acid will restore alanine (and leucine) uptakes. 

 Thus the polynucleotide is effective when the corresponding dinucleotide is not. 



Table 3. Effect of rna and polyadenylic acid supplements on amino acid incorporation 



BY DNAase-TREATED NUCLEI 



