204 SUBCELLULAR PARTICLES 



in the intact nucleus, the presence of deoxyribonucleic acid mediates amino acid 

 incorporation into protein, ATP synthesis, and the uptake of purines and 

 pyrimidines into nuclear ribonucleic acids. The role of DNA in these activities is 

 not yet clear, but from the DNA-substitution experiments which have been car- 

 ried out with isolated nuclei, one can conclude that the polyanionic nature of the 

 DNA plays a large part in its activity. The need for large negatively charged 

 molecules as DNA substitutes, and the inhibitory action of polycationic com- 

 pounds such as polylysine, suggest a correlation between negative electrical charge 

 and the biochemical activity of the nucleus. 



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