DEWITT STETTEN, JR. 



tions to synthesize fatty acids is far less if they are derived from 

 diabetic rather than normal animals, and these findings are 

 compatible with what is known to happen in the intact animal 

 (19). It is probably incorrect, however, to refer to such prepara- 

 tions as "diabetic liver slices." If by "diabetes" is meant the 

 relative or absolute deficiency of insulin, then all liver slices, 

 regardless of source, are necessarily diabetic. The difference 

 between isolated livers from diabetic and nondiabetic animals 

 is chronological, not diagnostic. Slices prepared from livers of 

 pancreatectomized or alloxanized animals have been deprived 

 of their source of insulin, have been "diabetic," for a somewhat 

 longer period of time than have similar slices prepared from 

 tissues of a normal animal. 



References 



1. Bernstein, C, Jr., J. B. Kirsner, and W. J. Turner, J. Lab. Clin. Med., 

 23, 938 (1938). 



2. Bornstein, J., and C. R. Park, J. Biol. Chem., 205, 503 (1953). 



3. Boxer, G. E., and D. Stetten, Jr., J. Biol. Chem., 153, 607 (1944). 



4. Chernick, S. S., I. L. Chaikoff, E. J. Masoro, and E. Isaeff, J. Biol. Chem.. 

 186, 527 (1950). 



5. Cori, G. T., Harvey Lectures, Ser. 48, 145 (1954). 



6. Harrison, D. C, Biochem. J. {London), 26, 1295 (1932). 



7. Ingle, D. J., E. H. Morley, and D. Stetten, Jr., Am. J. Physiol., 182, 

 263 (1955). 



8. Krahl, M. E., J. Biol. Chem., 200, 99 (1953). 



9. Levine, R., M. S. Goldstein, B. Huddlestun, and S. P. Klein, Am. J. 

 Physiol., 163,70 (1950). 



10. Lundsgaard, E., Biochem. Z., 264, 209 (1933). 



11. McQuarrie, I., E. T. Bell, B. Zimmermann, and W. S. Wright, Federation 

 Proc, 9, 337 (1950). 



12. Marsh, J. B., and N. Haugaard, J. Clin. Invest., 31, 107 (1952). 



13. Mirsky, I. A., R. H. Broh-Kahn, G. Perisutti, and J. Brand, Arch. Bio- 

 chem., 20, 1 (1949). 



14. Patterson, W. B., and D. Stetten, Jr., Science, 109, 256 (1949). 



15. Pauling, L., Proc. Am. Phil. Soc, 96, 556 (1952). 



16. Price, W. H., C. F. Cori, and S. P. Colowick, J. Biol. Chem., 160, 633 

 (1945). 



17. Shaw, W., and S. Gurin, Arch. Biochem. andBiophys., 47, 220 (1953). 



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