Evelyn Anderson ^^k 



in table 4. It may be added that Harrop and his associates'- observed no 

 disturbance in carbohydrate metabolism in the adrenalectomized dogs Avliic h 

 survived for five months on salt therapy. 



6. Fat Metabolism 



That the salt-treated adrenalectomized rat is able to absorb fat from tlic in- 

 testinal tract as well as the normal animal has been shown by Clark and Wick,"' 

 and Barnes, Wick, Miller, and MacKay.=- Using radioactive phosphorus, Still- 

 man, Entenman, Anderson and Chaikoft"' were able to demonstrate that there 

 is no interference in the phosphorylation of fat in adrenalectomized lais 

 whether they be given salt or deprived of it. Moreover, Barnes, Miller and 

 Burr,'' using tagged fatty acids, have demonstrated that the intestinal ab- 

 sorption and the rates of incorporation of fatty acid into neutral fat and 

 phospholipids of the intestinal mucosa were similar in normal rats and adre- 

 nalectomized rats given salt solution to drink. However, the rate of entrance of 

 the tagged fatty acid into the neutral fat of the liver was interfered with in 

 adrenalectomized rats; salt solution did not restore this function to normal. 

 The above findings are not entirely in accord with the recent reports of Ba- 

 vetta, Hallman, Deuel and Greeley,"' who studied the rate of absorption of 

 hydrogenated cotton-seed oil and found a decrease of 24 per cent in the rate 

 of absorption of fat in the salt-treated adrenalectomized rat and a decrease 

 of 38 per cent in the untreated adrenalectomized rat. It is apparent that salt 

 therapy is beneficial for fat absorption in the adrenalectomized animal, bm 

 the degree of restoration seems to vary with conditions of the experiment. 



7. Urinary Nitrogen and Survival TijMe of the Fasted Salt-Treated 



Adrenalectomized Animal 



When the adrenalectomized rat is fasted the nitrogen excreted in the mine is 

 about 25 per cent less than that of normal rats fasted a similar period (Evans™). 

 This is associated with a marked fall in blood sugar and in liver glycogen. 

 Long, Katzin and Fry'" have shown that the giving of adrenal cortical hor- 

 mone to fasted adrenalectomized rats increases the urinary nitrogen excretion 

 to a level above that excreted by fasted normal rats, and it also raises the 

 blood-sugar level and the liver glycogen stores. From these findings the de- 

 duction is made that one of the properties of the adrenal cortical hormone is 

 to stimulate protein catabolism. Anderson and Joseph' have found that 1.0 

 per cent sodium chloride solution to drink has a beneficial efi'ect upon the 

 fasted adrenalectomized rat both by increasing the survival jjcriod during the 

 fast and also by increasing the urinary nitrogen excretion. A sunnnar\ ol liu 

 findings is given in table 5. Male rats 10 weeks of age and weighing approxi- 

 mately 200 gm. were started on a fast immediately following operation. The 

 rats of the control group were subjected to a sham adrenalectomy in -whidi 

 the adrenal gland was merely manipulated with forceps. The rats in the ex- 

 perimental gioups were adrenalectomized. One group received only tap water 



