Reese, Koneff, and Wainman 4^7 



There are other difTexences in the cytoplasm distinguishable in the early 

 stages of development which enable one to differentiate the two types of 

 basophil. The cytoplasm of the thyroidectomy basophil is usually more baso- 

 philic than that of the castration cell. The cytoplasm of these cells contains 

 coarse floccular basophilic material which becomes more and more apparent 

 as the vacuoles increase in size and is subsequently forced into a small space 

 near the nucleus. 



The cytoplasm of young castration basophils, thotigh it may be alveolar in 

 structure, is more uniform than the thyroidectomy basophil and is more finely 

 granular. In the Nassonov-Kalotchev preparations, the cytoplasm of the cas- 

 tration basophil is generally less osmiophilic than is that of the thyroidec- 

 tomy cell. 



There are noteworthy changes in the Golgi apparatus in the two groups of 

 basophils other than the changes in position already mentioned. These are 

 the changes in size, shape, and internal structure of the Golgi apparatus. The 

 Golgi apparatus is a satisfactory indicator in the normal rat hypophysis of the 

 strain to which any individual cell belongs. Severinghaus' first stressed the im- 

 portance of this fact, and results from this laboratory are in agreement with 

 his findings. This does not, however, mean that the position of the Golgi 

 apparatus has equivalent value as an indicator of cell type after physiological 

 or pathological changes. Such conditions may alter the relation of the Golgi 

 apparatus to the nucleus and even the finer morphology of the apparatus. 

 Under such circumstances, if the Golgi apparatus only were used as the single 

 criterion, great difficulty might be experienced in determining whether a 

 cell belonged to the acidophil or basophil strain. In the thyroidectomy baso- 

 phil, the Golgi apparatus is so altered that it may bear little resemblance to 

 the classic description which Sever inghaus has given of that organelle in 

 normal basophil. At least some of these modifications which the Golgi 

 apparatus undergoes are coincident with changes in the cytoplasm, and doubt- 

 less result from such changes. As the Golgi apparatus is crowded to^vard the 

 nucleus, the originally rounded apparatus comes to lie as a cap against the 

 nucleus (pi. i, figs. 7 and 8). That we are dealing here with a thyroidectomy 

 basophil and not an enlarged acidophil is easily proved by the application of 

 a suitable counterstain, such as the Mallory-Azan to positive impregnations 

 of the Golgi apparatus. 



It will be remembered that in the castration basophil the relation of the 

 Golgi apparatus to the nucleus is quite different from that in the thyroidec- 

 tomy basophil. As Guyer and Glaus' noted, the Golgi apparatus not uncom- 

 monly lies between the nucleus and the enlarging vacuole (pi. 1, fig. 13). 

 This relationship is, however, far from invariable, the Golgi body very fre- 

 quently coming to lie side by side with the nucleus, both bordering the 

 vacuole (pi. 1, figs. 11, 12, and 14); in other words, the vacuole often begins 

 in a portion of the cytoplasm quite distant from the nucleus and Golgi ap- 



