^y 



104 SCIENTIFIC REPORTS OF THE AGRICULTURAL RESEARCH 



(ii) Eggs were obtained in two out of four experiments 

 tried with shed goat's bloods (sweetened with 

 cane sugar). With a view to studying the effects 

 of the different constituents of blood, attempts 

 were made to separate out the serum from the 

 shed blood by means of a centrifuge but this did 

 not succeed, probably on account of failure to 

 prevent coagulation. 



(Hi) Willstatter has recently confirmed the analogy 

 that was believed to exist between chlorophyll 

 and haemoglobin, but no eggs have hitherto been 

 laid by mosquitos fed with sweetened plant 

 juice. 



(iv) Mosquitos enclosed with tender plants (from 

 which they could suck the juice if they would) 

 died within four days without ovipositing. 



(«) In view of Goeldi's opinion that ' honey and other 

 sweet substances have an inhibitory or neutral 

 influence on reproduction,' saccharine and gly- 

 cerine in various strengths were offered to the 

 mosquitos but they refused even to taste any of 

 the liquids. 



" (6) A large number of experiments was carried out on 

 the behaviour of bed-bugs in a varying temperature. 

 Healthy adult bed-bugs were enclosed in very small air-tight 

 tubes designed to contain the least possible amount of air, 

 and the tubes containing the bugs were immersed in water 

 kept at a fixed temperature by means of electric current. 

 The experiments necessitated sustained observation in order 

 to discriminate between apparent and real death. The main 

 issue of the experiments was the establishment of the fact 

 that a two minutes exposure to a temperature of 52°C. is 

 generally sufficient to kill the bed-bug. 



" (7) In July 1919 the following were discovered in the 

 hole of an old tree : — 



(i) A large number of adult sand-flies (PMebotomus 

 sp. near minutus), 



