MICHIGAN ACADEMY OF SCIENCE. 



83 



TABLE I. 



I was not at all certain that the five lots, which showed no contamina- 

 tion in the foregoing experiments, would show the same results again, 

 if treated in a similar manner. For this purpose three lots of each 

 were set up and allowed to incubate. The results are given in Table II. 

 .Three of the five failed to show up sterile three times in succession, 

 while two did. That means that apparently only two lots out of the 

 original twenty-five could be depended upon as being sterile when treated 

 according to Table I. Of these two lots, one was Lupine and the other 

 was Mustard. 



TABLE II. 



Since the decisive failure to obtain sterile seeds, shown in Tables I 

 and II, might reasonably be expected to make the technique appear open 

 to criticism, to say the least, it was thought advisable to set up a set 

 of control cultures by means of the apparatus. After the apparatus had 

 been sterilized in the autoclave, it was attached to the aspirator and 



