32 ENZYMES 



Exercise V 



EQUIPMENT 



Per 2 students 



24 test tubes (small or medium); 8 more if possible 



8 dropping bottles 



1-ml pipet 



2 5-ml pipets 



50-ml graduate 



6 6" medicine droppers 



2 beakers suitable for 0°C and 100°C water baths 



potato 



porcelain spot plate 



cheese cloth (10" X 20") 



Per 8 students 



fresh beef heart 



mineral oil 



50-ml dropping bottle succinate solution (0.5 M, adj. 



to pH 7.5) 



50-ml dropping bottle malonate solution (1 M, adj. 



to pH 7.5) 



50-ml dropping bottle 0.01 % methylene blue solution 



50-ml dropping bottle iodine and potassium iodide 



solution (0.01 M) 



50-ml dropping bottle 0.5% starch (boiled) in 25% 



NaCl 



50-ml dropping bottle glucose (0.01 M) 



50-ml dropping bottle glucose-1-phosphate (0.01 M) 



50-ml dropping bottle KH2PO4 (0.2 M) 



50-ml dropping bottle 0.2% soluble starch 



50-ml dropping bottle sodium fluoride (0.01 A') 



50-ml dropping bottle Mcllvaine buffers (0.1 M) at 



pH 3.4, 5.0, 6.8, and 8.0 



vial of pHydrion paper 



Per laboratory 



water baths at 0°C, 37°C, and 100°C 

 chewing gum (1 stick per student) 

 Waring blendor 

 clinical centrifuge 



