90 PERMEABILITY AND ACTIVE TRANSPORT 



Exercise XVII 



(3) 1.22% CaCb (0.11 M) 



(4) 3.82% MgS04 • 7H2O (0.154 M) 



(5) phosphate buffer (0.1 M, pH 7.4) (17.8 gm of 

 Na2HP04 • 2H2O + 20 ml NHCl diluted to 1 

 liter) 



Use 100 parts of solution 1 ; 4 parts of solution 2; 

 3 parts of solution 3; 1 part of solution 4; 20 parts 

 of solution 5. Add glucose to make 20-mA/ (0.36%) 

 and 50-mM (0.90%) solutions just before using. 



The solution should be stirred while adding the 

 phosphate to prevent precipitation. 



dinitrophenol, saturated solution 



prepared slides of intestine 



prepared slides of kidney 



large water baths at 100°C may be used instead of 

 individual ones 



