Oi'ginally communicated in Kgl. Dannkc Videnskaberne-s Selskah. Biologiske 



Meddelelser, 15, 7 (1940) 



37. RATE OF RENEWAL OF THE ACID SOLI BLE 



ORGANIC PHOSPHORUS COMPOUNDS IN THE ORGANS 



AND THE BLOOD OF THE RABBIT 



G. Hevesv and L. Hahn 



From the Institute of Theoretical Physics, University of Copenhagen 



In a paper published recently in these Pioceeclings^i\ the rate of renewal 

 of the phosphatide molecules present in various organs of the rabbit 

 and other animals was discussed. In the present publication, data on the 

 rate of new formation of acid soluble phosphorus compounds are commu- 

 nicated. The acid soluble organic P compounds represent a great variety 

 of chemically very different bodies: esters as, for example, hexosephos- 

 phate, nucleotide compounds as adenosintriphosphate, phosphagen, and 

 other compounds. These compounds^^) ^j-g renewed at a comparatively 

 fast rate in the organs in contradistinction to the phosphatides and 

 desoxyribo nudeoproteins^^) Furthermore, while the rate of new forma- 

 tion of the phosphatides in the circulation is almost negligible, the 

 acid soluble phosphorus compounds are renewed at a remarkable rate in 

 the corpuscles. These facts justify the consideration of the acid soluble 

 phosphorus compounds from our view-point as a definite group of the 

 phosphorus compounds present in the body, 



EXPERIMENTAL METHOD 



Labelled P as sodium phosphate was administered by intravenous 

 or subcutaneous injection to rabbits all through the experiment in order 

 to keep the activity of the plasma inorganic P at a constant level. After 

 the lapse of some hours or days, the animal was killed by bleeding. 

 The fresh organs were placed in liquid air and w-ere extracted immedia- 

 tely with cold 10 per cent trichloroacetic acid. The inorganic phosphate 



^1^ G. Hevesy and L. Hahn, Kgl. Danske Vidensk. Sehkah. Biol. Medd. 15' 

 5 (1940). 



^-^ With the exception of adenylic acid [T. Korzybski and J. K. Pakxas, 

 Z. physiol. Chem. 255, 195 (1938)] and, possibly, of other not yd known n>inor 

 components of the acid soluble P mixture. 



^■''^ L. Hahn and G. Hevesy, Nature, April (>, 1940. 



"24 Heresy 



