374 ADVENTURES IN RADIOISOTOPE RESEARCH 



clearly be found to a larger extent incorporated in hexosemonophos- 

 phate molecules than those P atoms the activity of which corresponds 

 to an early stage of the experiment. 



Let us consider active hexosemonophosphate molecules which were 

 formed during the first stage of the experiment and which were again 

 newly formed during the last minute of the experiment. If the second 



Table 6. — Specific Activity of Acid Soluble P Fkactions 

 Extracted from the Corpuscles of the Rabbit 



Rabbits II, III, and IV (average) 

 Intravenous injection during 4 hours 



Time ot hydrolysis in 1 n H„S04 at 100" 



Specific activity 



at the end of the 



experiment 



Inorganic P (present as such in the corpxiscles) 



— 7 min 



7—100 min 



7 min — 12 hours 



Non-hydrolysed in the course of 12 hours (residue) 

 Non-hydrolysed in the course of 24 hours (residue) 



100 

 100 

 100 

 100 



87 

 77 



process were not forthcoming, we should find molecules of small activity; 

 if the opposite were the case, we should find the molecules to be strongly 

 active. When calculating the fraction of the hexosemonophosphate 

 molecules which were newly formed (once or several times) during the 

 experiment from the ratio 



specific activity of h exosemonophosphate P a t the end of the experiment 

 ~ average specific activity of inorganic P during the experiment 



we overestimate the percentage of hexosemonophosphate which was 

 renewed during the experiment. This will be especially the case if the 



ratio 



rate of renewal 



rate of intrusion 



is large, as, for example, in the case of the corpuscles. 

 When calculating from the ratio 



specific activity of organic P at the end of the experi ment 

 average specific activity of inorganic P during the experiment 



the extent of renewal of the acid soluble P mixture in the corpuscles, 

 we arrive at a value of 199 per cent (see Table I). Such a calculation, 

 for reasons stated above, supplies the upper hmit of the extent of renewal. 

 The lower limit is given by the ratio: 



