CIKC'LLATION OF PHOSPHORIS IX THE KllOG 



399 



slightly, the average being 108, taking the end value to be 100. The 

 values obtained for the specific activity of the tissue P are seen in Table 

 20 and Fig. 3. 



In some eases, very low values were obtained for the distribution 

 ratio of IuIjcHchI phosphate between plasma and muscle tissue. The fact 



200- 



150 - 





o 

 o 



o 100- 



u 



•> 

 a. 



o 

 o 



50- 



Gasrrocnemius 



Tibia epiphysis 

 Tibio diaphysis 



Time in hours 

 Fig. 3. Specific activity of tissue P. 



that in these experiments frogs kept through the winter were used, the 

 experiment being carried out in the spring, suggests the explanation 

 that poor circulation may be responsible for the low values obtained. 



Table 20. — Activity of Different Fractions of the Frog 1 Hour and 4 Hours, 

 Respectively, after the start of the Experiment 



Temp.: 22° 



Time 



1 hour 



Fraction 



4 hours 



Plasma 



Gastrocnemius 

 Epiphysis .... 

 Diaphysis .... 



Plasma 



Gastrocnemius 

 Epiphysis .... 

 Diaphysis .... 



Fresh weight 

 in mf'm 



P content 

 in mgm 



Activity 



per mgm 



fresh weight 



98.7 



861.8 



94.1 



67.6 



1267.5 



812.4 



38.8 



60.1 



0.00344 

 1.3.5.5 

 6.100 

 6.830 



0.044 

 1.300 

 3.61 

 6.410 



116(1) 

 32.7 

 224 

 235 



100 



93.5 

 755 



578 



Specific 



activity 



of P 



116 

 0.72 

 0.12 

 0.08 



100 

 2.03 

 0.28 

 0.19 



<•> Taking the 4 hours value to be 100. 



