INCORPORATION OF LAB. ACETATE CARBON ("C) INTO TISSUE FRACTIONS 409^ 



of acetate in a 100 gm rat amounting according to Block and Ritten- 

 BERGandPiHLe^a/. (1, 3) to 15-20 mM. 



The effects of X-rays on animal tissue was found, in experiments in 

 which labelled acetate was administered to mice, to influence markedly 

 acetate metabolism (4). This observation induced the investigation of 

 Ihe effects of metabolic inhibitors and accelerators on acetate meta- 

 bolism. 



Previous work with mice carried out by Ruyssen, Beeckmans and 

 one of us (5) showed an increased incorporation of ^^C in the urethano 

 injected animals. 



In the present investigation IIk^ effect of a metabolic ac^celerator, that 

 of dinitro-cyclo-pentylphenol (D.P.P.) was studied on ^^C incorporation 

 into the total tissue and the total fat of various organs of the mouse. We 

 choose dinitro-cyclo-pentylphenol as IIeymans and Casier (6) found 

 this compound to stimulate cellular metabolism to a larger extent than 

 any other dinitro-compound studied. 



Dinitro-cyclo-pentylphenol being a metabolic accelerator its effect 

 on cellular metabolism can be expected to reflect itself in the rate of 

 i^C incorporation into tissue fractions. 



As shown by different authors, oxygen consumption is increased 

 after administration of 2.4-dinitrophenol and similar compounds. 



Terada and Tainer (7) injected subcutaneously 20 mgm of 2—4 dini- 

 trophenol perkgm to 33 gm. rats and found an increase of about 25% 

 in the oxygen consumption 20 min after injection. After the lapse of 

 2 hours, normal oxygen consumption was again observed. In the case 

 of adult rats the same effect could be obtained by injection of 15 mgm 

 per kgm body weight. 



A slight stimulation of oxygen consumption was observed by Loomis 

 and LiPMANN (8) in their experiments in which the effect of small amounts 

 of dinitro-phenol on kidney homogenate metabolism was studied and a 

 marked lowering of phosphorylation observed. The inhibition of coup- 

 ling between oxidative processes and the formation of high energy bonds 

 was found by these authors to be a conspicuous effect of dinitro-phenol. 



EXPERIMENTS 



Five groups of five adult mice each, were injected with 0.3 cc. of a 

 solution containing 0.16% of sodium dinitro-cyclo-pentylphenol . (20 

 mgm for 1 kgm of animal). 



Five groups of controls were injected at the same time with 0.3 cc. 

 of a physiological sodium-chloride solution. 



Ten minuten afterwards all groups were injected intraperitoneally 

 with 3 l^t- of sodium acetate labelled in the carboxyl group. 



