INCORPORATION OF LAB. ACETATE CARBON ("C) INTO TISSUE FRACTIONS 411 



C 



O 

 <_) 



2000 



Liver 



Conrrol 



Di nit rocyc I openryl phenol 



t500 - 



000 



500- 



60 mm. 



Fig. 1. Effect of dinitro-cyclo-pentylphenol on the incorporation of 

 i*C into total fats (a) and total tissue (b) of the liver of the mouse, 



"C injected as CHgWCOONa. 



is metabolized at a slower rate than acetate and correspondingly in the 

 left part of the curve the acetate values are higher, in the right part of the 

 curve the succinate values are higher (2). 



A similar trend as indicated by the curves plotted in Fig. la is shown 

 by the curves seen in Fig. 16 in which the specific activity of the dry 

 liver tissue is plotted as function of time, both for controls and D.P.P. 

 injected animals. The ^^C-content of the liver tissue except in a very 

 early phase is mainly due to its fatty components but some is still pre- 

 sent in acetic acid and other acid soluble tissue components, and to a 

 very restricted extent in glycogen and in proteins. That the absolute 

 i^C content of the total tissue is much smaller than that of the fatty 

 components, is due to the dilution of highly active ftitty acids ])y large 

 amounts of slightly active tissue components. 



