INTERACTION OF PLASMA PHOSPHATE WITH THE PHOSPHOKL'S COMPOUNDS 49 L 



Table 8. — Parts in Million ok the Total Activity Injected Subcutaniously 

 Present in 1 mgm P Extracted from the Plasma and Corpuscles of Human. 



.Subjects 



a) Boy 



Sample taken after 120 miii 



Plasma phosphate 201 



Corpuscle total acid soluble P 57 



Sample taken after 7 days 



Plasma phosphate 10 



Plasma phosphatides^^ 14 



Corpuscle phosphatides 6 



b) Young female 



Sample taken after 130 min 



Plasma phosphate 480 



Corpuscles inorganic phosphate -j- hydrolysable ester P (hydrolysed for 



7 min at 100" in 1 n HCl) 87 



Corpuscles non-hydrolysable ester P 62 



Sample taken after 1 day 



Plasma phosphate 105 



Corpuscles total acid soluble 173 



Plasma phosphatide 31 



Corpuscles phosphatide 2.3- 



c) Old female 



Sample taken after 140 min 



Plasma phosphate 224 



Corpuscles inorganic phosphate -\- hydrolysable ester P (hydrolysed for 



7 min at 100° in 1 n HCl) 61 



Corpuscles non-hydrolysable ester 34 



Sample taken after 1 day 



Plasma phosphatide 7.5 



Corpuscles phosphatide 1.3 



Sample taken after 8 days 



Plasma phosphate 10 



Plasma phosphatide(i) 16 



Corpuscles phosphatide 7 



<•' We witness here an example of the case discussed in detail in former papers (for example Hahn and 

 Hevesy, Skatid. Arch. Phys. 77, 148 (1937) in which, due to the rapid exchange between bone phosphate 

 and plasma phosphate the active inorganic P is renewed from the plasma at a rapid rate while the removal 

 of the labelled phosphatide P is a much slower one. Some of the phosphatide molecules were formed from 

 a highly active plasma and were still present after a lapse of 7 days, while the greater part of the inorganic 

 P disappeared at this time from the plasma. Similar cases can also be found wlien investigating the acid 

 soluble P. 



Summary 



The rate at which labelled phosphate ions added to tlic plasma of rabbit 

 blood penetrate into the corpuscles was determined. A comparison of the specific 

 activity (activity per mgm P) of the inorganic P and of the est or P extracted 

 from the corpuscles suggests that the penetration of the labelled inorganic phos- 

 phate into the corpuscles is a comparatively slow process while the process in 

 which the labelled inorganic phosphate is incorporated into the easily hydrolysable 

 organic compounds of the corpuscles is a fast one. 



