EATE OF PENETRATION OF IONS INTO ERYTHROCYTES 501 



Q 



of e~Ri\ and thus on the temperature coefficient will be very much 

 larger if Q has a larger value than in the opposite case. 



Q 



For example, if ^^p, =^ K^^ the replacement of T by T/2 will result 



Q 

 in a reduction of the value of e^lvF from 4.5 • Id ^ to 2.1 • 10 ^, thuS 



Q 



to less than 1/5000 of the initial value while, if is taken to be = 1, 



RT 



Q 

 a corresponding change in T will result in a decrease of e~lrf from 0.37 



to 0.14, only. 



Assuming S = 1, the heat of activation of the penetration of phos- 

 phate (Q) can be calculated from the equation 



and as— — was found to be ^ 14, Q works out to be 15000 cal. 



INCORPORATION OF 32p nyxo ORGANIC COMPOUNDS IN BLOOD 



HEMOLYSATE 



As we stated above (p. 496), the rate of incorporation of ^sp into some 

 of the P compounds of the corpuscles is faster than the rate of penetration 

 of 32p through the corpuscle membrane. It is, thus, the rate of the last 

 mentioned slower process which determines the rate of interchange of 

 corpuscle P and plasma P as long as the specific activity of the cor- 

 puscle inorganic P remains behind the specific activity of the plasma 

 inorganic P. This statement is based on the fact that, w^hile the specific 

 activity of the corpuscle pyrophosphate P, for example, some time 

 after the start of the experiment not much differs from the specific 

 activity of the corpuscle inorganic P, 1 mgm of corpuscle inorganic P 

 is found to be much less active than 1 mgm of plasma inorganic P. This 

 conclusion involves the assumption that the fraction secured from the 

 de-proteinated corpuscles as inorganic phosphate was actually present 

 as such in the corpuscles and not as a constituent of a not yet known 

 labile P compound. This assumption may probably be correct. The 

 distribution coefficient of free phosphate between plasma and corpuscles 

 can be expected not to differ much from the distribution coefficient of 

 chloride and other free anions between plasma and corpuscles. Thus, 

 1 gm of corpuscle can to be expected to contain somewhat less than half 



