504 ADVENTURES IN EADIOISOTOPE RESEARCH 



about I as many labelled organic P compounds were synthesized 

 as at 37°. 



The lowering of the rate of formation of labelled adenosintriphosphate 

 in the hemolysate is possibly due partly or wholly to destruction of 

 cozymase taking place in the hemolysate. In a hemolysate of the cor- 

 puscles of horse blood Lennerstrand (1941) found, 3 hours after 

 hemolysis, a destruction of 40 per cent of the cozymase present in the 

 corpuscles. Lennerstrand found also that the increase of the inor- 

 ganic phosphate content of the hemolysate is due at least partly to 

 dephosphorylation of adenosintriphosphate. The composition of a mix- 

 ture of hemolysate, cozymase and adenosintriphosphate was found, 

 however, not to change. 



RuNNSTROM, Lennerstrand et al. (Lennerstrand, 1941) observed 

 in a hemolysate of corpuscles of horse blood a synthesis of organic 

 phosphate occurring at the expense of the oxydation of hexosedi- 

 phosphate. The reaction was found to require the presence of 

 diphosphodinucleotide, coenzyme and methylenblue or another dye 

 which acts as oxygen carrier. While clismutation and phosphorylation 

 were found to take place in the hemolysate, all processes consecutive to 

 the formation of monophosphoglyceric acid were found to be absent. 



The intermediary formation of inorganic phosphate is a normal 

 step in the phosphorylation circuit going on in the corpuscles. In the 

 intact corpuscle the inorganic phosphate split off is soon incorporated 

 into another organic molecule and its splitting off is thus hidden from 

 observation, except from using an isotopic indicator which reveals that 

 we are faced with a dynamic equilibrium. If the phosphorylation mecha- 

 nism is disturbed in the corpuscle or in the hemolysate, incorporation 

 of phosphate into organic compounds takes place at a reduced rate only 

 and an accumulation of free phosphate takes place in the blood. 



EFFECT OF POISONS ON THE ENZYMES RESPONSIBLE FOR THE 

 SYNTHESIS OF ORGANIC P COMPOUNDS IN THE CORPUSCLES 



In normal corpuscles, the penetration of ^^P into the corpuscle is 

 followed by an incorporation into organic molecules which, in turn, 

 give off nonlabelled P. By this process, the corpuscle inorganic P is 

 prevented from obtaining for quite a while a high specific activity. 

 If, however, a formation of organic P compounds is hindered, and this 

 should be the case in a poisoned corpuscle, the ^^P migrating into the 

 corpuscle would remain in the inorganic P fraction and this fraction 

 would soon become strongly active. 



We failed to inhibit the process of resynthesis by adding fluoride to 

 rabbit blood, as seen in Table 9. 2 ce. blood +0.5 cc. 0.2 mol sodium 



