RATE OF PEXETR.A.TION OF IONS INTO ERYTHROCYTES 



505 



TaBI.K 9. — DlSTRIBt'TION OF ^'P BETWEEN PlASMA 



.\ND COHPr.SCLES .\FTER 159 MIN IN THE ABSENCE 



AND PRE.SKNfE OF FlT'ORIDE 



fluoride solution were first shaken at 37° for 87minutes. ^^p -yy^s then 

 added and the blood was kept for further 159 minutes at 37°. In the 

 control experiments, the sodium fluoride solution was replaced by Rin- 

 ger's solution. Investigating phosphorylation in the hemolysate of the 

 corpuscle of the horse, Lennerstrand (1940) found that addition of 

 fluoride to the hemolysate does not inhibit phosphorylation, though 

 the rate of phosphorylation gets somewhat reduced. 



The addition of KCN to the blood, however, had a marked though 

 highly varying effect on the system of resynthesis. Rabbit blood was 

 shaken with different volumes (see Table 10) of an isotonic potassium 

 cyanide solution (pH = 7.5 — 8.0) at 37° for some time previous to 

 addition of labelled phosphate of negligible w-eight. As seen in Table 10^ 

 the corpuscles treated in this way took up less labelled phosphate than 

 the corpuscles which were not treated with cyanide. Furthermore, the 

 distribution of ^^p between the different phosphorus compounds present 

 in the corpuscles was different in the controls and in the cyanide treated 

 erythrocytes. In the corpuscles treated with cyanide, most of the ^sp 

 present was in the inorganic fractions. From this result follows that it 

 is not so much the permeability of the corpuscles which is influenced 

 by the presence of cyanide as the phosphorylation process occurring in 

 the corpuscles.' 



Table 10. — Uptake of ^^p by the Corpuscles of the Rabbit at 37° after 

 Treatment With Cyanide Solution at 37° 



