514 ADVENTURES IN RADIOISOTOPE RESEARCH 



Summary 



The red corpuscles contain much larger amounts of acid soluble phosphorus 

 compounds than the plasma. The difference is only maintained as long as an 

 active process, an alternative phosphorylation and dephosphorylation, takes 

 place in the corpuscles, a process which was studied by measuring the rate of 

 penetration of labelled phosphorus into the erythrocytes and the rate of incorpora- 

 tion of 32p into the acid soluble P compounds present in the corpuscles. 



While labelled phosphate penetrates at about the same rate into the corpuscles 

 of the human and the rabbit, it enters at a much slower rate the nucleated cor- 

 puscles of the hen and the frog. 



The amount of labelled phosphate which penetrates into the corpuscles of 

 the rabbit at 0° was found to make out about ^ of the amount found at 37°. 



Incorporation of labelled phosphate into acid soluble P compounds, thus alter- 

 native dephosphorylation and phosphorylation, was found to go on in the blood 

 hemolysate as well, though at a slower rate than in the intact corpuscles. 



A lowering of the temperature from 37° to 0° reduces the amount of labelled 

 P incorporated into organic P compounds of the hemolysate to ^. 



The addition of KCN to blood reduces the formation of organic P compounds 

 in the corpuscle markedly. The rate of penetration of phosphate into the 

 corpuscles was also measured in accumulation experiments in which a part of 

 the plasma chloride was replaced by phosphate and the amount of phosphate 

 which left the plasma determined at different intervals. 



The difference between the interchange of labelled and non-labelled ions, present 

 in the plasma and the corpuscles respectively, and the accumulation of ions in 

 the corpuscles after raising the ionic concentration of the plasma is discussed 

 and it is shown that only interchange experiments supply a direct measure of 

 the hindrance of a phase boundary to the passage of ions. 



By using ^ap and ^sQl, respectively, as indicators the percentage of plasma 

 phosphate which penetrates per unit time into the corpuscles of the rabbit was 

 found to be at least 100 times smaller than the percentage of plasma chloride 

 penetrating into the corpuscles. The rate of penetration of sodium into the cor- 

 puscles of the dog using ^^Na as a tracer, was found to be slower than the rate 

 of intrusion of phosphate. 



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