515 



COMlMKTs'T ON PAPERS 49 aiid 50 



The first investigations (paper 49) on the intrusion rate of P'^ into red corpuseles 

 brought out that even after several hours of incubation of blood nx the presence 

 of labelled sodium phosphate the specific activity of the corpuscular inorganic 

 P lags ver^• much behind the specific activity of the plasma morganic P. After 

 ■1 very short time, however, the specific activity of the labile acid-soluble P of 

 the corpuscles does not differ much from that of their inorganic P. This was inter- 

 preted as indicating a fairly slow penetration of P^^ into the eorpusc-les followed 

 bv a rapid interaction with the labile P of the acid-soluble P compounds present 

 in these In more recent years Gourley and also Gerlach et ah measured 

 specific activity values of the labile P of ATP of the red corpuscles which were 

 found to be larger than the specific activity of the inorganic P isolated from them 

 These observations suggest the explanation that at least some of the plasma, in- 

 organic P is incorporated into corpuscle ATP prior to its having the opportumty 

 to be "diluted" by the inactive inorganic P of the corpuscles, the incorporation 

 thus taking place 'in or on the phase boundaries. The isolation of corpuscle P is 

 a difficult task, as a part of the small amounts of isolated inorganic P from the 

 corpuscles may be an artifact, due to a splitting of a minute fraction of the large 

 amounts of organic P present in the corpuscles in the course of the extraction 

 process The above mentioned results must therefore be carefully mterpreted 

 As mentioned on p. 365 the synthesis of ATP in or on the phase boundaries of 

 liver cells was made very probable by Sacks. 



D R H. Gourley, (1952) Arch. Biochem. Biophys. 40, 1. 



E. A. Gerlach, A. Fleckenstein and K. J. Freundt, Z. Physiol. Chcm. 263, 

 682 (1957) 



^BTx 



33^ 



