SULFUR 309 



lactating cow (S-13). About 30 mc was oxidized to SO2 and stored in 

 standard 550-ml S02-gas tanks. The silage was preserved in gastight 

 55-gal steel drums. The S^^Oo was allowed to flow into the drum to give 

 the desired treatment level. After storage, it was determined that no 

 free S^^02 was present, and the silage was fed to dairy cows, with sub- 

 sequent analysis of urine, feces, milk, and blood samples by standard 

 methods. 



Some commercially available S^Mabeled compounds are listed as fol- 

 lows (S-14) : 



Benzyl cysteine-S'^ Z-Methionine-S" 



Cell hydrolysates-S^^ {E. coli and S. cerevisiae) d/-Methionine-S" 



Cells-S'^ (E. coli and S. cerevisiae) Mustard gas-S'^ 



Cells-S'^ (S. cerevisiae and T. utilis) Penicillin G-S^^ 



L-Cystine-S" Pentothal-S^s 



2,4-Dinitrobenzene sulfenyl chloride-S^^ Phenothiazine-S" 



Ethionine-S^s Pipsyl chloride-S" 



Glutathione-S'^ Potassium ethyl xanthate-S" 



Glutathione-S^5 (oxidized) Potassium thiocyanate-S^* 



Heparin-S'^ Sucaryl-S^^ 



Homocystine-S^* Sulfanilic acid-S^^ 



Insulin-S35 Thiourea-S^s 



S-1. Walser, M., A. F. Reid, and D. W. Seldin: A Method of Counting Radiosulfur 



in Liquid Samples, and Its Application to the Determination of S^^ Excretion 



Following Injection of S^^04, Arch. Biochem. and Biophys., 45: 91-96 (1953). 

 S-2. Tarver, Harold: Radioactive Sulfur and Its Applications in Biology, Advances in 



Biol, and Med. Phys., 2: 281-311 (1951). 

 S-3. Everett, Newton B., and Barbara S. Simmons: The Distribution and Excretion 



of S'^ Sodium Sulfate in the Albino Rat, Arch. Biochem. and Biophys., 35: 152- 



156 (1952). 

 S-4. Ray, Francis E., and Alary F. Argus: Studies on the Metabolism, Distribution, 



and Excretion of 2-p-Toluenesulfonamidofluorene-S^* in the Rat, Cancer 



Research, 11: 783-787 (1951). 

 S-5. Alaass, Alfred, Franc C. Larson, and Edgar S. Gordon: The Distribution in 



Normal Tissues of Radioactive Sulfur Fed as Labeled Methionine, J. Biol. 



Cheni., 177: 209-216 (1949). 

 S-6. Mechlin, L. J., P. B. Pearson, C. A. Denton, and H. R. Bird: The Utilization of 



Sulfate Sulfur by the Laying Hen and Its Incorporation into Cystine, J. Biol. 



Chem., 205: 213-219 (1953). 

 S-7. "Methods of Analysis of the Association of Official Agricultural Chemists," 



6th ed., Association of Official Agricultural Chemists, Washington, D.C., 1945. 

 S-8. Young, L., M. Edson, and J. A. McCarter: The Measurement of Radioactive 



Sulphur ("S) in Biological Material, Biochem. J. London, 44: 179-185 (1949). 

 S-9. Dziewiatkowski, Dominic D.: Radioautographic Visualization of Sulfur-35 



Disposition in the Articular Cartilage and Bone of Suckling Rats Following 



Injection of Labeled Sodium Sulfate, J. Expll. Med., 93: 451-458 (1951). 

 S-10. Yarwood, C. E., and Louis Jacobson: Selective Absorption of Sulphur-35 by 



Fungus-infected Leaves, Nature, 165: 973 (1950). 



