AUTOUADIOGRAPHY 



331 



Table 7-2. Directions for Staining after Photographic Processing and 

 Characteristics of Autoradiograms of Mounted Tissue Sections 



(Continued) 



[Courtesy of Victor H. Witten and Vera Holmstrom, New Histologic Technics for 

 Autoradiography, Lab. Invest., 2: 368-375 (1953), Paul B. Iloeber, Inc., publisher.] 



are separated after exposure, then the section can be stained in the usual 

 way at that time, since any removal of radioactivity would have no effect. 

 The section can be stained before contact with the emulsion, but in this 

 case one must be sure that leaching does not occur. Therefore, with prep- 

 arations that consist of combined section and emulsion, staining is usually 

 done after development, and special attention must be paid to the prob- 

 lem of absorption of the stain by the emulsion. The thinner the emul- 

 sion, the less difficulty from this cause. 



Directions and characteristics of some of the stains used for autoradio- 

 grams of mounted tissue sections are presented in Table 7-2, as compiled 

 by Witten and Holmstrom (20). 



