PAPER CHKOMATOGKAPHY 



303 



and the inside exposed wooden surfaces are paraffined to resist tlie action 

 of solvents. The box must be reasonably vaportight when closed. Fish 

 aquarium tanks can be readily adapted for this purpose (8). Datta et al. 

 (14) have described a convenient frame for piocessing up to 12 sheets 



Class cover plate 



Staining dish 

 (Multiple^ 

 ten place) 



Glass cylinder 



Cylindrical 

 glass Jar 



Fig. 8-1. Simple assembly for development of paper-strip chromatograms with 

 descending solvent flow. [From \V. Joe Frierson and John W. Jones, Radioactive 

 Tracers in Paper Partition Chromatography of Inorganic Ions, Anal. Chem., 23: 1447- 

 1452 (1951).] 



simultaneously. The frame consists of two duralumin plates which are 

 connected by four removable rods, one at each corner; the plates are of the 

 same dimensions as the sheets to be used. The sheets of filter paper are 

 threaded onto the rods, with a 2-cm collar placed on the rods between each 

 sheet to keep the papers apart. In this case, a tray containing the solvent 

 is placed at the bottom of the box, and the frame plus filter paper stands 



