376 



RADIOISOTOPES IN BIOLOGY AND AGRICULTURE 



Table 8-2. Summary of Chromatographic Separation of Organic Substances 



Substance 



Adrenalin and related 

 compounds 



Aliphatic acids 



Amount 



Aliphatic acids 

 (a-keto) 



Alkaloids (atropine 



compounds) 

 Alkaloids (nicotine, 



pyrrolidine, 



pyridine) 

 Alkaloids (sparteine 



and genistein) 

 Amines 



Amino acids. 



Amino acids (dinitro- 

 phenyl derivatives) 



Amino alcohols . 



Anthraquinone 



pigments 

 Ascorbic acid. . . 



Chloromycetin. 



Choline, ethanola- 

 mine, serine 



Flavonoid pigments 



Hexachlorocyclo- 

 hexane isomers 

 Indicator dyes. . . . 



/3-Indoleacetic acid 

 and tryptophan 



Lignin oxidation 

 products 



Nicotinic acid and 

 related compounds 



Penicillin 



•i Mg 

 0.5-3 Mg 



5-10 us 



0.5-5 Mg 



2-5 Mg 



1-15 Mg 

 5-20 Mg 



30 Mg 



1-2 units 



Paper 



Whatman 1, Schlei- 

 cher and Schuell 

 597 



Whatman 1 



Durieux 122 



Whatman 



Whatman 1, What- 

 man 4, Schleicher 

 and Schuell 507 



Whatman 4 



Whatman 1 



Whatman 1, What- 

 man 4 



Whatman 1, What- 

 man 2, Whatman 

 4, Eaton and Dike- 

 man 613, Schlei- 

 cher and Schuell 

 112 



Paper treated with 

 acetic anhydride 



Whatman 1 



Whatman 1, What- 

 man 2, Whatman 

 4, Eaton and Dike- 

 man 613, Schlei- 

 cher and Schuell 

 112 



Solvents 



Aqueous phenol, 

 fi-butanol-HCl 



n-Butanol-acetic 

 acid, M-butanol- 

 ammonia, ethanol- 

 ammonia, CCh- 

 acetic acid 



re-Butanol-water- 

 propionic acid, 

 a-n-dimethylami- 

 noisobutyric 

 nitrile-n-butanol- 

 water 



?(-Butanol-HCl 



«-Butanol-HCl 



n-Butanol-acetic 

 acid-water 



Same as for amino 

 acids, m-cresol- 

 acetic acid-water, 

 n-butanol-glycol 

 monochlorohydrin- 

 NHjOH 



Phenol, m-cresol, 

 lutidine, collidine 

 mixtures, butanol, 

 acetone 



Chloroform— isopro- 

 panol-0.05 M K 

 benzoate, pro- 

 Ijanol-petroleum 

 ether, r?-butanol- 

 ethanol-water 



fi-Butanol-acetic 

 acid-water, 

 n-butanol-NH40H, 

 phenol-NH40H 



Petroleum ether- 

 methanol 



Butanol-acetic acid, 

 phenol-acetic acid 



Butanol-acetic acid- 

 water, butanol- 

 phenol-pyridine 



n-Butanol-glycol 

 monochlorohvdrin- 

 NH4OH, n-biita- 

 nol-acetic acid- 

 water 



Ethyl acetate, phenol 



>i-Hexane-acetic 

 anhydride 



Isopropanol, buta- 

 nol, aniyl alcohol 



Methylethylketone- 

 pyriiline-water 



Petroleum ether- 

 water, 7!-butyl 

 ether-water 



?i-Butanol-HCl- 

 water 



Ether- water, anhy- 

 drous ether 



Detection 



Potassium ferri- 

 cyanide, ninhydrin 



Bromophenol blue, 

 bromocresol green, 

 bromothymol blue, 

 bromocresol purple 



o-Phenylenediamine, 

 semicarbazide 



Bismuth subnitrate- 



KI reagents 

 As the chlorohydrates 



Ninhydrin, Erlich's 

 reagent, quinone, 

 iodine 



Ninhydrin, iodine, 

 permanganate, 

 orcinol 



Ninhydrin 



Magnesium acetate 



2,6-Dichlorophenol- 



indophenol 

 SnClj reagent and 



P-dimethylamino- 



benzaldehyde 



Ninhydrin, iodine 



Fluorescence, alcohol 

 AICI3, lead acetate, 

 N&1CO3. FeCh 



FeS04 



Dilute NaOH 



Cinnamic aldehyde- 



HCl 

 2,4-Dinitrophenyl- 



hydrazine 



Ninhydrin, phospho- 

 molylidic acid- 

 stannous chloride 



Microbiological 

 assay 



