236 CHLORINE 



study (Cl-4, Cl-5) about 50 nc in 10 to 15 ml of normal saline was injected 

 per patient. 



CP^ studies have been undertaken with plants grown in sand culture 

 with a nutrient solution consisting of 0.003 M Ca(NO?)2-iH20, 0.007 M 

 KNO,, 0.001 M MgS04-7H20, 0.001 M KH2PO4, 1 ppm Fe, 0.5 ppm B, 

 0.025 ppm Mn, and 0.25 ppm Zn (Cl-6). The applications of radioactiv- 

 ity were as follows: One microcurie CP^ was poured on the surface of a 

 sand culture containing a mature tomato plant and flushed in with 1 liter 

 of nutrient solution; 1 /xc Cl'^^ was added as above to a sand culture con- 

 taining four alfalfa plants at 10 days before harvest; lima-bean plants 

 were treated with 0.05 yuc CP® added daily to the nutrient solution for 

 20 days before harvest; a mature corn plant was treated with 2 fxc CP^ at 

 10 days before harvest; and a solution containing 0.005 ^c/ml of CP* and 

 a wetting agent was painted daily for 14 to 21 days on the leaves of various 

 plant species. The plant tissue was measured for CP^ by grinding the 

 oven-dried material to 60 mesh and spreading 50 mg over the bottom of a 

 metal planchet. Counting was done with a 1.82-mg/cm'' mica-end- 

 window tube. Losses of activity were noted when ashing temperatures 

 above 300°C were employed, and the direct measurement of the dried 

 material appeared to be the one of choice. 



Cl-l. Franco, Vincent, and Bernard Klein: The Microdetermination of Chlorides in 

 Serum and Spinal Fluid, /. Lab. Clin. Med., 37: 950-954 (1951). 



Cl-2. Schales, O., and S. S. Schales: A Simple and Accurate Method for the Determina- 

 tion of Chloride in Biological Fluids, J. Biol. Chem., 140: 879 (1941). 



Cl-3. Burch, G. E., S. A. Threefoot, and C. T. Ray: Rates of Turnover and Biologic 

 Decay of Chloride and Chloride Space in the Dog Determined with the Long- 

 life Isotope, CP«, /. Lab. Clin. Med., 35: 331-347 (1950). 



Cl-4. Ray, C. T., G. E. Burch, and S. A. Threefoot: Biologic Decay Rates of Chloride 

 in Normal and Diseased Man, Determined with Long-lived Radiochloride, CP^, 

 ./. Lab. Clin. Med., 39: 673-696 (1952). 



Cl-5. Threefoot, S. A., G. E. Burch, and C. T. Ray: Chloride "Space" and Total 

 Exchanging Chloride in Man Measured with Long-life Radiochloride, CP*, 

 /. Lab. Clin. Med., 42: 16-33 (1953). 



Cl-6. Toth, S. J., and A. E. Kretschmer: Plant Studies with Radioactive Chlorine, 

 Soil Sci., 77: 293-302 (1954). 



