130 



Fig. 28. Running time of myotonic goats. Abscissa: days. Ordinate: 

 running time in seconds. Arrows: injection of thymus extract. Arrows 

 with circle: injection of saline. For details see text 



tained in 5 ml the extractive matter of 150 g frozen calf thymus, 

 which contained the fraction not precipitated isoelectrically, was 

 not thrown down by methanol but was precipitated by an excess 

 of acetone.^ The results of this experiment are reproduced in Fig. 

 28. The external conditions were not favorable and so the normal 



^ Prep. 1. 16 Kg of frozen calf thymus glands were minced, suspended in 

 28 liters of water, and sent through a colloid mill. After the addition of 

 4l4 ml of glacial acetic acid, the suspension was diluted with 30 liters of 

 water. The undissolved material was separated on a Sharpies centrifuge and 



