76 SELECTION AND PREPARATION OF ORGANISMS 



of chlorophyll per liter. The final dark green suspension is stored in small 

 test tubes in the freezer at —36° C. At this storage temperature the 

 chloroplast fragments retain their photochemical activity for months. The 

 small tubes are removed from the freezer and thawed for use in experi- 

 ments as needed. 



This, then, is one method. Others will be found in the following refer- 

 ences. 



SELECTED REFERENCES 



Anonymous, Basic Care of Experimental Animals. Revised edition. 

 Prepared by the Animal Welfare Institute, 22 East 17th Street, New 

 York 3, New York. 1958. Useful information on housing, feeding, 

 handling, etc., with special emphasis on humane treatment of the 

 animals. Included are eight mammal species, and poultry, amphibians, 

 turtles, and fish. 



Arnon, D. I., "Cell-free photosynthesis and the energy conversion 

 process," in A Symposizim on Light and Life, William D. McElroy 

 and Bentley Glass, eds. Baltimore: Johns Hopkins Press, 1961. pp. 

 489-569. A review which discusses (among other things) the prob- 

 lems in the use of isolated chloroplasts. Provides references to most 

 of the methods of preparing chloroplasts. 



Bonner, James. 1959. Protein synthesis and the control of plant 

 processes. American Journal of Botany 46:58-62. Bonner comments 

 on the use of isolated parts of cells, and discusses specifically the prep- 

 aration and use of microsomes (now called ribosomes) from plants. 



Brachet, Jean, and Alfred E. Mirsky, eds.. The Cell: Biochemistry, 

 Physiology, Morphology, vol. I-V. New York: Academic Press, 

 1959-61. A comprehensive, authoritative reference work: Vol. I 

 (1959)-Methods and problems in biology; vol. II (1961)-Cells 

 and their component parts; vol. Ill (1961)— Meiosis and mitosis; vol. 

 IV and V (1 960-6 1)-Specialized cells. 



Cass, Jules S., Irene R. Campbell, and Liili Lange, A Gxiide to Pro- 

 duction, Care and Use of Laboratory Animals: An Annotated Bib- 

 liography. Federation Proceedings 19(4) Part III, Supplement No. 

 6. 1960. A list of references to literature with abstracts of the papers. 

 Information on normal animals, disease, nutrition, breeding programs, 

 and most of the other topics that might concern the experimentalist. 



