62 



F. F. NORD, M. BIER 



VOL. 12 (1953) 



TABLE V 



SEDIMENTATION BEHAVIOUR OF TRYPSIN (Tr) AND TRIPSINOGEN (TrCn) 



REMARKS 



Enzyme preparation: SF = salt free; Cr = crystalline. 



Buffers: A = o.i M NaCI + o.oi M HCl; B = o.i M NaCl + o.i M HCl; C = o.i M NajB^O^; 

 D = 0.01 M NajB^O, + o.iM NaCl; E = 0.05 M CaCla + 0.1 M CHgCOgNa — CH3CO2H; F = 0.05M 

 MgClg t- O.I M CHjCOjNa — CHjCO^H; G = 0.1 M CHjCO^Na — CHjCOgH. 



Experiments: No. i-g: Average sedimentation rate at pH 2.4 = 2.40 ± 0.07 S; No. 12-13: After 

 24 and 48 h incubation. Heavier components appear, with s > 20 S; No. 16: After 24 hr. self-digestion. 

 Slower sedimenting components appear; No. 14-18: Average sedimentation rate at pH 8 = 2.52 

 ± 0.04 5; No. 19-24: Average sedimentation rate at pH 5.2 in presence of CaClj = 2.56 ± 0.02 5; 

 No. 25-36: Sedimentation rate in absence of calcium ions is strongly temperature dependent. 



References p. 66. 



