i66 



A. L. SCHADE 



VOL. 12 (1953) 



often moribund. From Fig. i it is evident that the concentrations of the three enzymes 

 in the blood plasmas of the tumorous mice were increased over the normal controls. 

 Aldolase and a-glycerophosphate dehydrogenase concentrations rose steeply to 13 and 

 20 times, respectively, the normal values with increasing age of the tumor while the 

 isomerase remained essentially constant over the observed tumor growth period of 6 to 

 13 days at the 6 to 7 fold elevated level. The ascitic fluids obtained from ascitic tumor 

 cell suspensions 6, 9, and 13 days after inoculation into the hosts all showed enzyme levels 

 significantly greater than those found in the companion blood plasmas. 



Fig. 2 summarizes typical data obtained with the Ehrlich ascites carcinoma in strain 

 C albino mice from an experiment performed in a manner parallel to that employed with 



8 



<b 



I 



75 



60 



45 



30 



15 



ED Blood plasma 

 ■ Ascitic fluid 



Aldolase 



Isomerase a-Olycerophosphafe 



dehydrogenase 



11 



5 9 13 5 9 13 5 9 13 



Days after inoculation 



Fig. 2. Ehrlich ascites carcinoma. Aldolase, isomerase, and a-glyccrophosphate dehydrogenase con- 

 centrations in blood plasma of normal strain C albino mice, and in blood plasma and ascitic lluid of 

 strain C albino mice 5, g, and 13 days after intraperitoneal inoculation with Ehrlich ascites carcinoma. 



the dba thymoma and described above. Under our conditions, the Ehrlich ascites 

 carcinoma developed at such a rate that 0.5 to i.o ml of ascitic cell suspension was con- 

 veniently available one day earlier than with the dba thymoma. Death of the hosts of 

 both tumors, however, ensued after the same lapse of time, 13 to 14 days on the average. 

 As is seen from Fig. 2, the level of the several enzymes in the blood plasmas of the tumor 

 bearing mice compared to that of the normal was sharply elevated in a way qualitatively 

 similar to that found with the dba thymoma. Quantitatively the rise was much less, 

 being only 2 to 3 fold for aldolase and isomerase and 6 fold for the a-glyccrophosphate 

 dehydrogenase. With the exccjition of the concentration of the dehydrogenase in the 



References p. lyi. 



