VOL. 12 (1953) ASCITIC TUMORS AND THEIR HOST'S BLOOD PLASMAS 



167 



ascitic fluids of the 9- and 13-day old tumors, the levels of activity of all three enzymes 

 in the ascitic fluids were greater than those of the assiociated plasmas. Repeated experi- 

 ments with the Ehrlich ascites carcinoma showed the a-glycerophosphate dehydrogenase 

 concentrations in both the ascitic fluid and the blood plasma to be comparable or 

 slightly in favour of the blood plasma while the other two enzymes were always in greater 

 concentrations in the ascitic fluids. 



After aliquots of the several ascitic cell suspensions of both the dba thymoma and 

 the Ehrlich ascites carcinoma were incubated in nitrogen and in oxygen for 90 minutes, 



400 



^350 

 s 



o 

 o 



^300 

 250 



200- 



Fresh ascitic fluid 



Og- incubated ascitic fluid 



Ng-incubafed ascitic fluid 



n 'Glycerophospfiafe 

 dehydrogenase 



Aldolase 





Isomerase 



W 



i. 



6 9 13 

 Days after Inoculation 



Fig. 3. dba ascites thymoma. Aldolase, isomerase, and a-glyceropliosphate dehydrogenase concentra- 

 tic fluid and in ascitic fluid incubated inNj and in O^, of dba i 

 after intraperitoneal inoculation with dba ascites thymoma. 



tions in fresh ascitic fluid and in ascitic fluid incubated inNj and in Oj, of dba mice 6, 9, and 13 days 



the cells were centrifuged off and the enzyme levels of the ascitic fluids were determined. 

 The results obtained were then compared with the values earlier established for the 

 aldolase, isomerase, and a-glycerophosphate dehydrogenase activity of the fresh ascitic 

 fluids. 



Fig. 3 presents the data recorded for the dba thymoma. With this tumor there was 

 no significant increase in the aldolase or isomerase activity of the ascitic fluids as a re- 

 sult of the anaerobic incubation of the ascitic cell suspensions. The incubation in oxygen 

 effected a slight decrease in aldolase activity of the fluids from older tumor cell suspen- 

 sions while it appeared to affect little, if at all, their isomerase activity. Only with 

 References p. lyi. 



