i68 



A. L. SCHADE 



VOL. 12 (1953) 



respect to the a-glycerophosphate dehydrogenase was there a suggestion that incubation 

 in nitrogen, under our conditions, led to an increase in the activity of this enzyme in the 

 ascitic fluids. However, since comparable increases in activity were observed in those 

 fluids from cell suspensions incubated in oxygen, the small enzymatic activity rise {ca. 

 17%) was not attributable to anaerobiosis. 



Fig. 4 summarizes the data obtained with the Ehrlich ascites carcinoma maintained 

 n nitrogen and oxygen atmospheres. The results contrast strikingly with those ob- 



120 



105 



^90 



Aldolase 



Fresh ascitic fluid 



0^' incubated ascitic fluid 



N^- incubated ascitic fluid 



a - Olycerophosptia te 

 dehydrogenase 



Isomerase 



Fi£ 



13 5 9 13 



Days after inoculation 



4. Ehrlich ascites carcinoma. Aldolase, isomerase, and a-glycerophosphate dehydrogenase 



concentrations in fresh ascitic fluid and in ascitic fluid incubated in Nj and in Og, of strain C albino 

 mice 5, 9, and 13 days after intraperitoneal inoculation with Ehrlich ascites carcinoma. 



served for the dba thymoma. Here, with the Ehrlich carcinoma, incubation of the ascites 

 cell suspensions in nitrogen led to large increases in the aldolase concentration of the 

 ascitic fluids as already reported by Warburg and Hiepler^. Comparable increases 

 were observed in the a-glycerophosphate dehydrogenase concentrations, while definite, 

 though smaller, rises in the isomerase levels also occurred. Incubation in oxygen re- 

 sulted in some rise in the concentration of all three enzymes in the ascitic fluids but of 

 less magnitude. In all our experiments with the Ehrlich ascites tumor, anaerobic incuba- 

 tion of the cell suspensions always led to mcrease in these enzymic activities of the ascitic 

 fluid while aerobic incubation resulted in slight reduction, in no change, or in some 

 increase in activity. 

 References p. iji. 



