336 



M. WOODS, K. WIGHT, J. HUNTER, D. BURK 



VOL. 12 (1953) 



approximately 40° C for 13 to 17 hours prior to sacrifice (Table II), the percentage 

 response to insulin was much greater than in tumor slices from comparable animals 

 maintained for the same period at t8 to 20° C (47 vs 7%). In the table the (^co values 



TABLE II 



Geo VALUES OBTAINED IN FOUR GROUPS OF S-QI MELANOMAS FROM MICE EXPOSED TO HIGH (35° TO 

 40° C) OR LOW (18° TO 20° C) TEMPERATURES FOR INDICATED TIME PERIODS. IN EACH GROUP THE 

 TUMORS HAVE BEEN ARRANGED IN ORDER OF ASCENDING MAGNITUDE OF Q^^ OF THE CONTROLS 

 (without added insulin). KREBS-RINGER-BICARBONATE, 0.625% DEXTROSE, O.03 M KHCO3 AND 



95% Ng, 5% CO.^ IN ALL CASES 



» Experiments run from February 27 to March ig, 1953. 

 ^^ 4 units low-zinc insulin plus i.i y zinc per ml. 

 '^ Experiments run from May 14-28, 1953. 



'' Crystalline zinc-insulin (0.8 to 4 imits per ml) in all but second tumor at 35'' C wliich rcociv'cd 

 4 units low-zinc insulin per ml. 



for the controls at all temperatures (minus added insulin) have been arranged in order 

 of ascending magnitude. The general range of values in the 40° C series is obviously 

 lower than in the corresponding low temperature series (average 11.52 vs 16. q5). On the 

 other hand in the presence of insulin the range of values in 40° C tumors is similar to the 

 range of values in the low temperature series (average 16.95 vs 18.10). The data suggest 

 that exposure of the animals to 40" C resulted in a lowering (average 32%) of the tumor 

 (2c6 '^^^ that this could be largely restored by insulin. In contrast to these results, slices 

 References p. 346. 



