848 



ADVENTURES IX RADIOISOTOPE RESEARCH 



a set of centrifuge tubes containing saturated Ba(0H)2. 3 bottles each containing 

 50 ml solution pro,-*;d to be sufficient to absorb all COg. The centrifuged BaCOg 

 precipitate was wasned with COg-free water and ethanol, dried first in a vacuum 

 exsiccator, then by heating to 105°. 



C I 



o 

 u 



X 



T3 



V 



a 



X 



01 



CO 



o 



U 



o 



CO 



4400 



4000- 



3600- 



3200 



2800 - 



2400 



2000 - 



1600 - 



1200 - 



800 - 



400- 



X »= Previous 



I irradintion 



After irradiation 

 with 1500 r 



0- 



, ' 1 1 1 ' 1 r 



20 40 60 80 100 120 140 160 



Time in minutes following injection 



Fig. 1. Effect of irradiation on the exhalation of "COg by labelled 



ethyl alcohol injected mice. 



To each mouse 0.050 to 0.100 ml of a 20 per cent ethyl alcohol solution labelled 

 by addition of 1 to Sfigm alcohol- 1 -i*C were administered by intraperitoneal injection. 

 The exhalatory ^^COg of the same group of mice was collected at intervals of 10 

 min first, previous to irradiation and again after the lapse of two days and also 

 after exposure to a dose of 1500 r. In both cases ethanol was injected in each experi- 

 ment before the collection of the exhaled COg had started, and within i^ hr of the 

 termination of irradiation which took 30 min. The Roentgen tube apphed was 

 run with 180 kV and 7 milliamp. In some of our experiments the controls were 

 injected in intervals of 2 days. This was made possible by the fact that the specific 



