OriginaUy published in Acta Physiol. Scand. 32, 339 (1954). 



61. EFFECT OF ADRENALINE ON THE INTERACTION 

 BETWEEN PLASMA AND TISSUE CONSTITUENTS 



G. Hevesy and G. Dal Santo 



From the Institute of Biochemistry and Organic Chemistry of the University 

 of Stockhohn and the Pharmacological Institution of Karolinska Institutet 



A LARGE number of mice were injected intraperitoneally with labelled 

 phosphate, partly after previous administration of adrenaline, partly 

 without, and 15 min later the distribution of 32p between Hver, muscles, 

 skeleton, and blood plasma was determined. The ^sp content of the 

 plasma was found to be markedly depressed in the adrenaline injected 

 mice. Besides the rate of extrusion of the labelled phosphate from the 

 circulating plasma, its rate of resorption into the circulation may be influ- 

 enced as well. We injected a tracer amount of labelled phosphate into the 

 circulation of the rabbit with the aim of eliminating the last mentioned 

 effect and followed its rate of disappearance. Adrenaline was found to 

 increase very markedly the rate of extrusion of 32p from the vascular 

 bed also in this case. 



The rate at which phosphate passes the capillary wall is a very high 

 one. In order to investigate the effect of adrenahne on a slowly penetrat- 

 ing compound we studied the rate of extrusion of labelled iron injected 

 intravenously as iron /5-globuhn in the controls and in the adrenaline 

 injected rabbits. Administration of adrenaline was found to accelerate 

 the exodus of the slowly disappearing ^^Fe from the circulation. We 

 determined furthermore the rate of exodus of radiosodium from the 

 circulation. 



EXPERIMENTAL 



In each of our first thirteen experiments, 10 controls and 10 adrenaline admi- 

 nistered mice of about 18 gram were injected with 0.1 ml saline containing 0.3 

 microcurie of ^ap present as carrier-free sodium phosphate. 15 min later, the 

 mice were decapitated and the ^ap content of dry plasma, hver, femur, and gastroc- 

 nemius tissue of the same weight of controls and adrenaline administered mice 

 was compared. 2 to 20 microgram of adrenaline chloride dissolved in 0.1 ml 

 saline were injected subcutaneously to every second mouse 10—40 min before 

 administration of ^ap. The controls were injected at a corresponding time with 

 0.1 mi saline which did not contain adrenaline. In the later series of these experi- 

 ments, 10 microgram of adrenaline dissolved in 0.1 ml of saline were injected 



