HAEMOGLOBIN' IX XUCLEAR FRACTIOX OF THE LIVER 



637 



(b) Experiments with rabbits 



The amount of ^^Fe present 4 hr after injection in one ^gm iron of the 

 non-extractable haemoglobin of the nuclear fraction of the rabbit liver 

 varied between 2x10"* and 6x10"*% of that injected. 



The iron content of the non-extractable liver nucleus fraction haemo- 

 globin varied between 0.5 and 3.8 /Ligm with a mean value of 1.9 //gm 

 per one g liver. 



I 



<s 

 O 



I 



<n 

 O 



I 

 o 



I 



ro 



O 



_u- 



+ 

 ro 

 O 



+ 

 O 



+ 

 O 



+ 

 CD 



o 



Circulating hemoglobin 



Liv5r nucleus 



f'-QCtion 



hemoglobin 



Total liver iron 



(D O 



•a 



O 3 



<e 



a> 



O (/I 



-• a 

 o 



Fig. 2. Effect of exposure of the rat to whole body irradiation on the 



incorporation of intraperitoneally injected ^^Fe as citrate into Uver 



fractions and the circulating haemoglobin. 



For the nuclear haemoglobin content of 1 gm of rat and rabbit liver 

 the corresponding figures are 0.38 mgm and 0.56 mgm. 



We also carried out experiments with pregnant rabbits 21 days after 

 mating. A plasma sample of the mother was labelled as described by 

 Ehrenstein e^a^(i^) The livers of the mothers and foetuses were investi- 

 gated 11 to 17 hr after labelling the maternal plasma. The results are seen 

 in Table 2. 



Table 1. — Ratio or Specific Activities of Iron Fractions 

 OF to 500 r Exposed and Control Rats 



