RIBOSENUCLEIC ACID IN THE JENSEN-SARCOMA OF THE RAT 



683 



To ascertain if the filtrates containing the ribosenucleotides are free 

 of phosphatides, we were shaking the two filtrates (A and B) with 2 

 volumes of ether and 0.2 volume of N 0.1 HCl to extract phosphatides 

 present. As seen above, the etheric phases contained a negligible amount 

 of P only. 



Table 2. — Results of Activity Measurements 

 (32P Content of Fractions) 



Sample 



Counts/niin. 

 of aliquote 

 precipitated 



Counts/min. 

 of total volume 



Oounts/min. 

 per mgm P 



Plasma inorg. P 



Sarcoma inorg. P 



A. 



Desoxyribosenucleic acid P ] 



Total P in filtrate after removal of desoxy j 



ribosenucleio acid I 



Inorganic P in filtrate 



Ribosenucleic acid P 



Residual fraction 



B. 



Desoxyribosenucleic acid P 



Total P in filtrate obtained after removal of 



desoxyribosenucleic acid 



Inorganic P in filtrate 



Ribosenucleic acid P 



Residual fraction 



461.5 



597.5 



260.5 



96.3 

 126.9 



11.7 



290.8 



121.2 

 101.5 



57.1 



1792.5 



651.3 



2890 

 846 

 204.4 

 14.6 



727 



3030 

 951 

 2079 

 71.4 



8758 

 6766 



147 



389 

 2745 



288 



135 



398 



2910 



279 



Specific Activity Ratios 



("Percentage New-formation Figures") 



Activity of 1 mgm desoxyribonucleic acid in percentage of activity 

 of 1 mg : 



A. Sarcoma inorganic P 2.18 



B. Sarcoma inorganic P 2.0 



A. Plasma inorganic P 1.68 



B. Plasma inorganic P 1.55 



Activity of 1 mgm ribosenucleic acid in percentage of activity of 1 mgm : 



A. Sarcoma inorganic P 4.27 



B. Sarcoma inorganic P 4.13 



A. Plasma inorganic P 3.29 



B. Plasma inorganic P 3.19 



