LABELLED SUBSTllATES IX EXZYMIC PROCESSES 



785 



The results are seen in Table 1. In the last column of this table the 



difference between the total acid-soluble P and the inorganic P is stated. 



From the figures of Table 1 follows, that a minor amount of the 



nucleic acid is not precipitated by addition of trichloracetic acid alone 



Table 1. — Effect of Plasma Phosphatase on 

 Desoxybibontjcleic Acid 



(it is stated that better results are obtained by adding besides trichlor- 

 acetic acid, hydrochloric acid as well)^^\ After the lapse of 2 hours, about 

 Ye of the phosphate was split off under the action of the plasma phospha- 

 tase, and after the lapse of 24 hours, most of the phosphate of the nucleic 

 acid was present as inorganic phosphate. Small amounts of organic 

 acid-soluble P were present as well. As described on p. 786 this frac- 

 tion is much increased when plasma phosphatase is replaced by in- 

 testinal mucosa phosphatase. 



Table 2. — Percentage Phosphate Split off 



FROM Nucleic Acid added to Plasma of Normal 



and of Jensen-Sarcoma Rats. T = 37° C 



(1^ 



G. Schmidt and S. Tannhauseb, J. Biol. Chem. 161, 83 (1945). 



50 Hevesy 



