1012 



ADVENTURES IN RADIOISOTOPE RESEARCH 



verted into glucose-6-phosphate the further steps lead either through 

 the phosphogluconate pathway (left) or through the triose phosphate 

 pathway (right). By applying "C labelled glucose he could demonstrate 



Table 3. — ^^p Content of the Virus Synthetised in tjn- 



LABELLED CeLLS KePT IN AN ACTIVE MEDIUM 



Radiactivity of virus synthetized in unlabelled cells in media 



containing ^^P 



that RNA is derived from the phosphogluconate path and that after 

 infection of the bacteria with the Tg bacteriophage the metabolic path 

 of glucose is switched over to the triose phospha'^e path. 



Glucose 



ATP 



■)Glucose-6-Phosphate« 

 TPN " 



'Fruorose 1,6 Diphosphate 



6-Phosphogluconolacrone 



ATP i 



Gluconate > 6-Phosphogluconare 



TPN 

 -CO2 



-Cz 



I 



Ribuiose >Ribulose-5-Phosphate^=;^^=^ Triose Phosphate 



J~ Jl CH3CH0I" 



D-arabinose Ajp ^1 J 



Ribose >Ribose-5-PhosphaTe Deoxyribose-5-Phosphare 



jr % 



Nucleoside Deoxynbonucleoside 



RNA DNA 



Fig. 12. — Alternative pathways of glucose metabolism prior to 



and after infection of E. coli. 



We discussed earlier the effect of insulin on glucose metabolism and 

 also its effect on the transformation of glucose into fatty acids. Here 

 two pathways are found as well, one being the so-called Emden — Meyerhof 

 pathway, the other involving oxydation of phosphogluconate. Experi- 

 ments with liver slices of diabetic rats treated with insulin brought out 

 that insulin treatment accelerates fatty acid formation from labelled 

 glucose through the Emden— Meyerhof pathway 70 times; through the 

 phosphogluconate pathway however, as much as 375 times. 



